Methylated DNA Quantification Kit (Colorimetric) from Abcam

Product Specs
ItemMethylated DNA Quantification Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab117128
Quantity48 TEST, 96 TEST
Sample TypeSaliva, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells
Sensitivity0.2 ng/well
Time4h 0m
ApplicationsCellular Assay
ReactivitySpecies independent
TypeQuantitative
Target5-methylcytosine

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Repetitive Transcranial Magnetic Stimulation Reduces Depressive-like Behaviors, Modifies Dendritic Plasticity, and Generates Global Epigenetic Changes in the Frontal Cortex and Hippocampus in a Rodent Model of Chronic StressCellsAugust 14, 2023Meneses-San Juan D, Lamas M, Ramírez-Rodríguez GB.was performed with the DNA samples using the kit “Methylated DNA Quantification colorimetric” (Cat: ab117128, Abcam). The absorbance of the reaction was measured at 450 nm with a multidetector (GloMax Discover,

(2) Early Development of Cardiac Fibrosis in Young Old-Father OffspringOxidative Medicine and Cellular LongevitySeptember 19, 2022A. Ismail, Y. Saliba, N. Faresstain (ThermoFisher Scientific, Waltham, MA, USA). Global cardiac methylation and hydroxymethylation (ab117128 and ab117130, Abcam, Cambridge, UK) were measured by the ELISA technique according to the manufacturer's

(3) Low mitochondrial DNA copy number induces chemotherapy resistance via epithelial-mesenchymal transition by DNA methylation in esophageal squamous cancer cellsJournal of Translational MedicineAugust 29, 2022Yuto Kubo et al.assay The overall DNA methylation in cells was analyzed using a methylated DNA quantification kit (ab117128, Abcam, Cambridge, UK), which quantifies global DNA methylation by specifically measuring the levels

(4) Primary cilia and SHH signaling impairments in human and mouse models of Parkinson's diseaseNature communicationsAugust 16, 2022Schmidt S, Luecken MD, Trümbach D, Hembach S, Niedermeier KM, Wenck N, Pflügler K, Stautner C, Böttcher A, Lickert H, et al.(Illumina). DNA methylation was quantified using the Methylated DNA Quantification Kit (Colorimetric) (Abcam, ab117128) according to the manufacturer’s instructions. An amount of 100 ng sample genomic DNA was used per reaction.

(5) Untargeted Metabolomics Showed Accumulation of One-Carbon Metabolites to Facilitate DNA Methylation during Extracellular Matrix Detachment of Cancer CellsMetabolitesMarch 21, 2022Suza Mohammad Nur, Mohammed Razeeth Shait Mohammed, Mazin A. Zamzami, Hani Choudhry, Aamir Ahmad, Bushra Ateeq, Irfan A. Rather, Mohammad Imran KhanGenomic DNA isolation Kit, Molequle On, Auckland, New Zealand). The 5mC kit was purchased from Abcam (lot no. ab117128, Abcam, Trumpington, UK). The extracted DNA was seeded for binding the DNA to the assay well. Then,

(6) Antioxidant and Antiaging Properties of a Novel Synergistic Nutraceutical Complex: Readouts from an In Cellulo Study and an In Vivo Prospective, Randomized TrialAntioxidants (Basel)February 26, 2022Sophia Athanasopoulou, Marianna Kapetanou, Michel Georges Magouritsas, Nikoletta Mougkolia, Polykseni Taouxidou, Michael Papacharalambous, Fotios Sakellaridis, Efstathios Gonosmeasurement of global DNA methylation status was performed by using the Global DNA methylation kit (ab117128, Abcam, Cambridge, UK), and for each sample, the input for the assay consisted of 100 ng of isolated

(7) The methylome of Biomphalaria glabrata and other mollusks: enduring modification of epigenetic landscape and phenotypic traits by a new DNA methylation inhibitorEpigenetics & ChromatinDecember 1, 2021Nelia Luviano, Marie Lopez, Fleur Gawehns, Cristian Chaparro, Paola B. Arimondo, Slavica Ivanovic, Patrice David, Koen Verhoeven, Céline Cosseau, Christoph Grunaupreprint [56]. ELISA-based 5mC quantification Methylated DNA Quantification Kit (Colorimetric) (Abcam, ab117128) was used to determine global 5mC level in isolated genomic samples of mollusk controls (n = 15 for

(8) Paternal low protein diet and the supplementation of methyl-donors impact fetal growth and placental development in micePlacentaJanuary 1, 2021Hannah L. Morgan, Arwa Aljumah, Charlène Rouillon, Adam J. Watkinsdiluted to 50 ng/μl. Global DNA methylation was quantified using the Methylated DNA Quantification Kit (ab117128, Abcam, UK) following manufacturer's instructions with plates read at 450 nm, 2 min from reaction end,

(9) YTHDC2-Mediated circYTHDC2 N6-Methyladenosine Modification Promotes Vascular Smooth Muscle Cells Dysfunction Through Inhibiting Ten-Eleven Translocation 2Frontiers in cardiovascular medicineJanuary 1, 2021Yuan J, Liu Y, Zhou L, Xue Y, Lu Z, Gan J.Nanodrop. Global DNA methylation was quantified using the methylated DNA Quantification (Colorimetric) Kit (ab117128, Abcam, UK) following manufacturer's instructions with plates read at 450 nm on a Bio-Rad microplate

(10) A Step Forward in Breast Cancer Research: From a Natural-Like Experimental Model to a Preliminary Photothermal ApproachInternational Journal of Molecular SciencesDecember 18, 2020Eduardo Costa, Tânia Ferreira-Gonçalves, Miguel Cardoso, João M. P. Coelho, Maria Manuela Gaspar, Pedro Faísca, Lia Ascensão, António S. Cabrita, Catarina Pinto Reis, Isabel V. Figueiredo9681 5. 18 of 28 DNA Methylation The ELISA-based “Methylated DNA Quantification Kit (Colorimetric)” (Abcam ab117128, Cambridge, UK) was used to quantify global 5mC DNA methylation content in control mammary tissue and

(11) Addressing the challenges of E-cigarette safety profiling by assessment of pulmonary toxicological response in bronchial and alveolar mucosa modelsScientific reportsNovember 24, 2020Ganguly K, Nordström A, Thimraj TA, Rahman M, Ramström M, Sompa SI, Lin EZ, O'Brien F, Koelmel J, Ernstgård L, et al.hydroxymethylation To assess the plausible epigenetic effect of E-smoke exposure, DNA methylation (ab117128, Abcam) and hydroxymethylation (ab117130, Abcam) was measured in the alv-ALI model (3 passages: 2 replicates/passage)

(12) Transcriptomic and Epigenetic Alterations in Dendritic Cells Correspond With Chronic Kidney Disease in Lupus NephritisFrontiers in ImmunologyAugust 27, 2019Anna Wardowska, Michał Komorniczak, Barbara Bułło-Piontecka, M. Alicja Dȩbska-Ślizień, Michał PikułaSpectrophotometer (Bio-Tek, USA). The ELISA-based Methylated DNA Quantification Kit (Colorimetric) (ab117128, Abcam, UK) was used to quantify global DNA methylation content in human dendritic cell subpopulations.

(13) Interplay between proinflammatory cytokines, miRNA, and tissue lesions in Anisakis-infected Sprague-Dawley ratsPLOS Neglected Tropical DiseasesMay 15, 2019Jerko Hrabar, Željka Trumbić, Ivana Bočina, Ivana Bušelić, Anamarija Vrbatović, Ivona Mladineopreviously. Global DNA methylation was quantified using a Methylated DNA Quantification Kit (Colorimetric) (ab117128, Abcam, Cambridge, UK), following the manufacturer’s protocol. The plate was read on a Microplate Photometer

(14) Loss of mdig expression enhances DNA and histone methylation and metastasis of aggressive breast cancerSignal Transduction and Targeted TherapyDecember 1, 2018Chitra Thakur, Bailing Chen, Lingzhi Li, Qian Zhang, Zeng-Quan Yang, Fei Chenquantification of global DNA methylation was performed using the Methylated DNA Quantification Kit (ab117128, Abcam, Cambridge, MA) and Hydroxymethylated DNA Quantification Kit (ab117130, Abcam Cambridge, MA)

(15) Determination of the presence of 5-methylcytosine in Paramecium tetraureliaPLOS ONEOctober 31, 2018Aditi Singh, Adrienne Vancura, Rafal K. Woycicki, Daniel J. Hogan, Alan G. Hendrick, Mariusz Nowacki5-methyl cytosines in total genomic DNA was done using EpiSeeker methylated DNA Quantification Kit (ab117128, Abcam). Briefly, DNA is bound to strip wells (provided with the kit) having a high DNA and the methylated

(16) Structural and mechanistic insights into UHRF1-mediated DNMT1 activation in the maintenance DNA methylationNucleic acids researchApril 6, 2018Li T, Wang L, Du Y, Xie S, Yang X, Lian F, Zhou Z, Qian C.DNA methylation quantification The ELISA-based ‘Methylated DNA Quantification Kit (Colorimetric)’ (Abcam, ab117128) was used to quantify global DNA methylation content in mouse ES cell lines. The assay was performed

(17) Cardiotrophic Growth Factor-Driven Induction of Human Muse Cells Into Cardiomyocyte-Like PhenotypeCell transplantationFebruary 1, 2018Amin M, Kushida Y, Wakao S, Kitada M, Tatsumi K, Dezawa M.relative percentage of DNA methylation was determined using the Methylated DNA Quantification Kit (cat. #ab117128; Abcam, Cambridge, UK). DNA Bisulfite Sequencing DNA was extracted from naive adherent Muse, naive

(18) Parental Exposure to Dim Light at Night Prior to Mating Alters Offspring Adaptive ImmunityScientific ReportsApril 1, 2017Yasmine M. Cissé, Kathryn L.G. Russart, Randy J. NelsonGlobal DNA methylation status was quantified using a Colorimetric Methylated DNA Quantification Kit (Ab117128, Abcam, Cambridge, UK) according to manufacturer instructions. Absolute quantities of methylated DNA

(19) Hypo-methylation mediates chromosomal instability in pancreatic NETEndocrine-Related CancerMarch 1, 2017I Marinoni, A Wiederkeher, T Wiedmer, S Pantasis, A Di Domenico, R Frank, E Vassella, A Schmitt, A Perrensamples was analyzed by its global methylation status using EpiSeeker methylated DNA Quantification Kit (ab117128, Abcam), according to the supplier’s instructions. Colorimetric measurements were done on an ELISA reader

(20) Djulis (Chenopodium formosanum Koidz.) Water Extract and Its Bioactive Components Ameliorate Dermal Damage in UVB-Irradiated Skin ModelsBioMed research internationalJanuary 1, 2016Hong YH, Huang YL, Liu YC, Tsai PJ.precancerous DNA-damaged cells to survive. We detected the degree of DNA methylation by a commercial kit (ab117128, Abcam) as the assay of DNA protection. The data show there was no change of percentage of 5-methylcytosine

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Methylated DNA Quantification Kit (Colorimetric) from Abcam

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Methylated DNA Quantification Kit (Colorimetric) from Abcam

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